Reverse transcriptionplugin-autotooltip__default plugin-autotooltip_bigRNA transcription: the process of RNA polymerase using the DNA sequence of a gene as a template to form an mRNA (in prokaryotes) or pre-mRNA (in eukaryotes). In most cases, “transcription” implies RNA transcription.: the formation of cDNAsplugin-autotooltip__default plugin-autotooltip_bigComplementary DNA (cDNA): DNA that has been reverse transcribed from spliced mRNA using reverse transcriptase. cDNA sequences are useful because they lack intron sequences and therefore contain information about the ORF of intron-containing eukaryotic genes. from mRNAsplugin-autotooltip__default plugin-autotooltip_bigmessenger RNA (mRNA): an RNA molecule that codes for protein. using reverse transcriptaseplugin-autotooltip__default plugin-autotooltip_bigReverse transcriptase: an enzyme that is usually isolated from retroviruses. It catalyzes the formation of cDNA using RNA (usually mRNA) as a template..