RNA sequencingplugin-autotooltip__default plugin-autotooltip_bigRNA sequencing (RNAseq): an experimental technique that sequences all the RNAs in a sample. It is based off of converting RNAs into cDNAs with reverse transcriptase, followed by Illumina sequencing. (RNAseqplugin-autotooltip__default plugin-autotooltip_bigRNA sequencing (RNAseq): an experimental technique that sequences all the RNAs in a sample. It is based off of converting RNAs into cDNAs with reverse transcriptase, followed by Illumina sequencing.): an experimental technique that sequencesplugin-autotooltip__default plugin-autotooltip_bigSequence: the precise order of monomers in a polymer. In DNA, it refers to the order of G, A, T, and C nucleotides. In RNA, it refers to the order of G, A, U, and C nucleotides. In proteins, it refers to the order of amino acids. all the RNAsplugin-autotooltip__default plugin-autotooltip_bigRNA sequencing (RNAseq): an experimental technique that sequences all the RNAs in a sample. It is based off of converting RNAs into cDNAs with reverse transcriptase, followed by Illumina sequencing. in a sample. It is based off of converting RNAsplugin-autotooltip__default plugin-autotooltip_bigRNA sequencing (RNAseq): an experimental technique that sequences all the RNAs in a sample. It is based off of converting RNAs into cDNAs with reverse transcriptase, followed by Illumina sequencing. into cDNAsplugin-autotooltip__default plugin-autotooltip_bigComplementary DNA (cDNA): DNA that has been reverse transcribed from spliced mRNA using reverse transcriptase. cDNA sequences are useful because they lack intron sequences and therefore contain information about the ORF of intron-containing eukaryotic genes. with reverse transcriptaseplugin-autotooltip__default plugin-autotooltip_bigReverse transcriptase: an enzyme that is usually isolated from retroviruses. It catalyzes the formation of cDNA using RNA (usually mRNA) as a template., followed by Illumina sequencingplugin-autotooltip__default plugin-autotooltip_bigIllumina sequencing: named after the company that developed this method, Illumina sequencing is currently the most widely used NGS technology for high throughput DNA sequencing..