Taq DNA polymeraseplugin-autotooltip__default plugin-autotooltip_bigDNA polymerase: an enzyme that is usually involved in DNA replication. This enzyme uses deoxyribonucleotides (dNTPs) and a primed template as a substrate to synthesize a new strand of ssDNA that pairs with its template to form dsDNA. There are many different kinds of DNA polymerase, but in this book we collectively refer to them as (pronounced “tack”; often just abbreviated as Taq polymerase) is a heat stable DNA polymeraseplugin-autotooltip__default plugin-autotooltip_bigDNA polymerase: an enzyme that is usually involved in DNA replication. This enzyme uses deoxyribonucleotides (dNTPs) and a primed template as a substrate to synthesize a new strand of ssDNA that pairs with its template to form dsDNA. There are many different kinds of DNA polymerase, but in this book we collectively refer to them as that retains functionailty even after repeated heating and cooling cycles used during the polymerase chain reactionplugin-autotooltip__default plugin-autotooltip_bigPolymerase chain reaction (PCR): An experimental technique invented by Kary Mullis used to exponentially amplify DNA in vitro. PCR made obtaining large quantities of DNA for analysis much faster and easier than using traditional cloning methods.. Standard enzymesplugin-autotooltip__default plugin-autotooltip_bigEnzyme: a macromolecule, usually a protein (but sometimes an RNA), that functions as a catalyst of some kind of biochemical reaction. will typically denature an lose activity after repeated heating and cooling. It is isolated from the bacteriumplugin-autotooltip__default plugin-autotooltip_bigBacteria: Single-celled organisms that also utilize DNA and the standard genetic code as all organisms on earth, but unlike eukaryotes do not have intracellular membranes and membrane-bound organelles. In this book we use bacteria and prokaryote interchangeably. Thermus aquaticus, which is found in high temperature deep sea thermal vents.