TNBGGA: The No Bullshit Guide to Genetic Analysis

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-<typo fs:x-large>Chapter 04. Chromosomes and sex linkage</typo> +<-chapter_03|Chapter 03^table_of_contents|Table of Contents^chapter_05|Chapter 05-> 
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 +<typo fs:x-large>Chapter 04. %%Chromosomes and sex linkage%%</typo> 
  
 Until now our analysis of genes has focused on defining genes based on phenotypic differences brought about by different alleles or by a direct test of function – the complementation test. In Chapters 4 and 5, our analysis will be concerned with tests of gene position.  Until now our analysis of genes has focused on defining genes based on phenotypic differences brought about by different alleles or by a direct test of function – the complementation test. In Chapters 4 and 5, our analysis will be concerned with tests of gene position. 
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 {{ :fruit-flies-red-and-white-eyes.jpg?400 |}}  {{ :fruit-flies-red-and-white-eyes.jpg?400 |}} 
 <caption> <caption>
-Wild type Drosophila melanogaster with red eyes (left) and the famous white mutant (right). Source: [[https://commons.wikimedia.org/wiki/File:Fruit-flies-red-and-white-eyes.jpg|Wikimedia]]. Licensing: [[https://creativecommons.org/licenses/by-sa/4.0/deed.en|CC BY-SA 4.0]].+Wild type //Drosophila melanogaster// with red eyes (left) and the famous white mutant (right). Source: [[https://commons.wikimedia.org/wiki/File:Fruit-flies-red-and-white-eyes.jpg|Wikimedia]]. Licensing: [[https://creativecommons.org/licenses/by-sa/4.0/deed.en|CC BY-SA 4.0]].
 </caption> </caption>
 </figure> </figure>
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 <WRAP center round box 60%> <WRAP center round box 60%>
-$$ \begin{aligned} P: white\text{ ♂} &\times \text{red ♀ (wildtype)}\\&\downarrow\\F1: \text{all} &\text{ red} \text{ (both ♂ and ♀)}\\+$$ \begin{aligned} P: white\text{ ♂} &\times \text{red eyes ♀ (wildtype)}\\&\downarrow\\F1: \text{all} &\text{ red} \text{ (both ♂ and ♀)}\\
 &\downarrow\\F2: \text{red}&:white = 3:1 \text{ (but only ♂ had white eyes)} \end{aligned}$$ &\downarrow\\F2: \text{red}&:white = 3:1 \text{ (but only ♂ had white eyes)} \end{aligned}$$
  
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-Morgan explained these unusual results by hypothesizing that the eye color gene white ($w$) is physically located on the sex chromosome $X$. Males only have one copy of the $X$ chromosome and females always get one copy of $X$ from their mother and one copy from their father. We can use modified symbols to show the genotypes that are directly associated, or linked, with the $X$ chromosome, and re-write Figure {{ref>Fig4}} as follows:+Morgan explained these unusual results by hypothesizing that the eye color gene $white$ ($w$) is physically located on the sex chromosome $X$. Males only have one copy of the $X$ chromosome and females always get one copy of $X$ from their mother and one copy from their father. We can use modified symbols to show the genotypes that are directly associated, or linked, with the $X$ chromosome, and re-write Figure {{ref>Fig4}} as follows:
      
 <figure Fig5>   <figure Fig5>  
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 In Figure {{ref>Fig5}}, each parent contributes one allele to the progeny; therefore, the wildtype allele for red eyes in female progeny is always inherited along with the $X^+$ chromosome from the father that carries the wild type $w^+$ allele (abbreviated as simply +), and the mutant $w$ allele is inherited from the $X^w$ chromosome from the mother. The fact that these individuals get two $X$ chromosomes (one from each parent) is what makes them females. Male progeny, on the other hand, are male because they get a $Y$ chromosome, and the only way to get a $Y$ chromosome is from their father; this means that they must get their $X$ chromosome from their mother, and their mother is homozygous $\frac{X^w}{X^w}$. Morgan named this phenomena sex linkage.  In Figure {{ref>Fig5}}, each parent contributes one allele to the progeny; therefore, the wildtype allele for red eyes in female progeny is always inherited along with the $X^+$ chromosome from the father that carries the wild type $w^+$ allele (abbreviated as simply +), and the mutant $w$ allele is inherited from the $X^w$ chromosome from the mother. The fact that these individuals get two $X$ chromosomes (one from each parent) is what makes them females. Male progeny, on the other hand, are male because they get a $Y$ chromosome, and the only way to get a $Y$ chromosome is from their father; this means that they must get their $X$ chromosome from their mother, and their mother is homozygous $\frac{X^w}{X^w}$. Morgan named this phenomena sex linkage. 
  
-Based on this discussion, we now have a clear definition of sex linkage in Drosophila: a gene is sex-linked if it is physically associated with a sex chromosome. The relevance of sex linkage is that this phenomena is what first allowed scientists to show that genes are associated with chromosomes. Morgan's experiments showed that at least one gene ($w$) is physically linked to the $X$ chromosome. In the next chapter, we will see that there are other Drosophila genes that are also sex linked. Subsequent experiments using Drosophila and other organisms showed that other genes are physically located on autosomes (any chromosome that is not a sex chromosome). Genes that are not sex-linked are located on autosomes and are also called autosomal genes. In the next chapter, we will also see that not only are genes physically linked to chromosomes, they also have defined physical positions on chromosomes.+Based on this discussion, we now have a clear definition of sex linkage in Drosophila: a gene is sex-linked if it is physically associated with a sex chromosome. The way a geneticist would say it is, "A gene is sex-linked if it maps to a sex chromosome".  The relevance of sex linkage is that this phenomena is what first allowed scientists to show that genes are associated with chromosomes. Morgan's experiments showed that at least one gene ($w$) is physically linked to the $X$ chromosome. In the next chapter, we will see that there are other Drosophila genes that are also sex linked. Subsequent experiments using Drosophila and other organisms showed that other genes are physically located on autosomes (any chromosome that is not a sex chromosome). Genes that are not sex-linked are located on autosomes and are also called autosomal genes. In the next chapter, we will also see that not only are genes physically linked to chromosomes, they also have defined physical positions on chromosomes.
  
 Finally, let's have some further discussion on genetic notation. Note that writing $X^w$ is redundant. Since the $X$ chromosome always pairs with the $Y$ chromosome during meiosis, the presence of the $Y$ chromosome automatically implies that any genes written together with the $Y$ chromosome in fractional notation must be on chromosome $X$. Furthermore, the male and female symbols are also redundant, since the presence of a $Y$ chromosome tells you everything you need to know about the sexes of the individuals in a cross where sex-linked genes are involved. Drosophila geneticists also use the $\rightharpoondown$ symbol to represent the $Y$ chromosome. We can therefore re-write Figure {{ref>Fig5}} as: Finally, let's have some further discussion on genetic notation. Note that writing $X^w$ is redundant. Since the $X$ chromosome always pairs with the $Y$ chromosome during meiosis, the presence of the $Y$ chromosome automatically implies that any genes written together with the $Y$ chromosome in fractional notation must be on chromosome $X$. Furthermore, the male and female symbols are also redundant, since the presence of a $Y$ chromosome tells you everything you need to know about the sexes of the individuals in a cross where sex-linked genes are involved. Drosophila geneticists also use the $\rightharpoondown$ symbol to represent the $Y$ chromosome. We can therefore re-write Figure {{ref>Fig5}} as:
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-A final note on sex linkage: many students are confused by sex linkage - they think of it as some kind of special case in genetics. Technically, it is indeed a special case. But it is best to think of sex linkage more as a general case of genetics. What sex linkage historically taught us is that genes are physically associated with chromosomes. It just so happens that some genes are on sex chromosomes, and those sex chromosomes also happen to determine sex. Since sex is easy to observe, it means that this fact that genes are associated with chromosomes just happened to be first discovered for genes on sex chromosomes. But the general statement that "genes are physically associated with chromosomes" is true for all genes and for all chromosomes. When thinking about sex linked genes and their inheritance, it can be easier to think about how these genes segregate with the $X$ and $Y$ chromosomes first, since chromosomes always follow the rules of meiosis. Then think about the sex of the offspring as a secondary thing. Consider chromosomes and genotypes and how they segregate first, then ask what the resulting phenotypes come from those genotypes second. Use this approach to think about all genes and chromosomes, including sex linked genes and sex chromosomes.+A final note on sex linkage: many students are confused by sex linkage - they think of it as some kind of special case in genetics. Technically, it is indeed a special case. But it is best to think of sex linkage more as a general case of genetics. What sex linkage historically taught us is that genes are physically associated with chromosomes. It just so happens that some genes are on sex chromosomes, and those sex chromosomes also happen to determine sex((Also note that just because a gene is sex-linked doesn't necessarily mean that this gene is involved in sex determination. There are lots of "regular" genes on the X chromosome that have nothing to do with sex determination.)). Since sex is easy to observe, it means that this fact that genes are associated with chromosomes just happened to be first discovered for genes on sex chromosomes. But the general statement that "genes are physically associated with chromosomes" is true for all genes and for all chromosomes. When thinking about sex linked genes and their inheritance, it can be easier to think about how these genes segregate with the $X$ and $Y$ chromosomes first, since chromosomes always follow the rules of meiosis. Then think about the sex of the offspring as a secondary thing. Consider chromosomes and genotypes and how they segregate first, then ask what the resulting phenotypes come from those genotypes second. Use this approach to think about all genes and chromosomes, including sex linked genes and sex chromosomes.
  
 ===== Questions and exercises ===== ===== Questions and exercises =====
chapter_04.1724880374.txt.gz · Last modified: 2024/08/28 14:26 by mike