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--- chapter_11 [2024/09/01 17:00] – [Stable regulatory circuits] mike
+++ chapter_11 [2025/04/07 20:58] (current) – mike
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-<typo fs:x-large>Chapter 11. Gene circuits and epistasis</typo>
+<-chapter_10|Chapter 10^table_of_contents|Table of Contents^chapter_12|Chapter 12->
+<typo fs:x-large>Chapter 11. %%Gene circuits and epistasis%%</typo>
 In [[chapter_10|Chapter 10]], we studied regulatory mechanisms in well-known //E. coli// operons to see how mutations in different elements of the system would behave in dominance tests and cis/trans tests. We also presented the information in reverse - we told you the answer first, then discussed how mutant phenotypes were interpreted.
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 </figure>
-Although loss of function mutations in genes for repressors or activators are generally the most common type of regulatory mutation, Table {{ref>Tab1}} will help you to interpret mutations in sites or more complicated mutations in proteins. With mutants in hand, you can potentially clone them by complementation as discussed in [[chapter_09|Chap. 09]]. You can then sequence your clones as discussed in [[chapter_08|Chap. 08]]. This will allow you identify the amino acid sequence of the protein/enzyme that carries out the function of the gene that is mutated in your mutants. This approach of discovering protein/enzyme function based on random mutants with interesting phenotypes is called forward genetics.
+Although loss of function mutations in genes for repressors or activators are generally the most common type of regulatory mutation, Table {{ref>Tab1}} will help you to interpret mutations in sites or more complicated mutations in proteins. With mutants in hand, you can potentially clone them by complementation as discussed in [[chapter_09|Chap. 09]]. You can then sequence your clones as discussed in [[chapter_08|Chap. 08]]. This will allow you to
+identify the amino acid sequence of the protein/enzyme that carries out the function of the gene that is mutated in your mutants. This approach of discovering protein/enzyme function based on random mutants with interesting phenotypes is called forward genetics.
 <table Tab1>
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 ===== Questions and exercises =====
-Exercise 1 (challenge question): Revisit [[chapter_02|Chapter 02]] Figure 3. Is it possible to analyze yeast $his$ mutants using epistasis? Why or why not? What question would you be answering with epistasis? What additional information might you need to know first? You may want to look up some classic genetic experiments by Beadle and Tatum using the bread mold Neurospora to help with answering this question.
+Exercise 1 (challenge question): Revisit [[chapter_02|Chapter 02]] Figure 3. Is it possible to analyze yeast $his$ mutants using epistasis? Why or why not? What question would you be answering with epistasis? What additional information might you need to know first? You may want to look up some classic genetic experiments by [[wp>One_gene–one_enzyme_hypothesis|Beadle and Tatum]] using the bread mold Neurospora to help with answering this question.