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--- chapter_11 [2024/09/01 17:03] – [Questions and exercises] mike
+++ chapter_11 [2025/04/07 20:58] (current) – mike
@@ Line 1: / Line 1: @@
-<typo fs:x-large>Chapter 11. Gene circuits and epistasis</typo>
+<-chapter_10|Chapter 10^table_of_contents|Table of Contents^chapter_12|Chapter 12->
+<typo fs:x-large>Chapter 11. %%Gene circuits and epistasis%%</typo>
 In [[chapter_10|Chapter 10]], we studied regulatory mechanisms in well-known //E. coli// operons to see how mutations in different elements of the system would behave in dominance tests and cis/trans tests. We also presented the information in reverse - we told you the answer first, then discussed how mutant phenotypes were interpreted.
@@ Line 15: / Line 17: @@
 </figure>
-Although loss of function mutations in genes for repressors or activators are generally the most common type of regulatory mutation, Table {{ref>Tab1}} will help you to interpret mutations in sites or more complicated mutations in proteins. With mutants in hand, you can potentially clone them by complementation as discussed in [[chapter_09|Chap. 09]]. You can then sequence your clones as discussed in [[chapter_08|Chap. 08]]. This will allow you identify the amino acid sequence of the protein/enzyme that carries out the function of the gene that is mutated in your mutants. This approach of discovering protein/enzyme function based on random mutants with interesting phenotypes is called forward genetics.
+Although loss of function mutations in genes for repressors or activators are generally the most common type of regulatory mutation, Table {{ref>Tab1}} will help you to interpret mutations in sites or more complicated mutations in proteins. With mutants in hand, you can potentially clone them by complementation as discussed in [[chapter_09|Chap. 09]]. You can then sequence your clones as discussed in [[chapter_08|Chap. 08]]. This will allow you to
+identify the amino acid sequence of the protein/enzyme that carries out the function of the gene that is mutated in your mutants. This approach of discovering protein/enzyme function based on random mutants with interesting phenotypes is called forward genetics.
 <table Tab1>