https://genetics.academic.csusb.edu/ 2026-09-06T06:44:14+00:00 https://genetics.academic.csusb.edu/ https://genetics.academic.csusb.edu/_media/wiki:logo.png text/html 2025-05-17T04:53:15+00:00 mike (mike@undisclosed.example.com) https://genetics.academic.csusb.edu/chapter_16?rev=1747457595&do=diff Chapter 15Table of ContentsChapter 17 Chapter 16. Gene knockouts in multicellular model organisms Totipotent mouse embryonic stem cells Knocking out mouse genes, or deliberately targeting a specific gene for mutation (as opposed to creating random mutants with a chemical mutagen), is a much more complex process than making $X$$X$$X$$neo^R$$neo^R$$neo^R$$tk^{HSV}$$tk^{HSV}$$tk^{HSV}$$neo^R$$X$$tk^{HSV}$$tk^{HSV}$$tk^{HSV}$$tk^{HSV}$$X$$neo^R$$X$$X$ text/html 2025-05-17T04:52:38+00:00 mike (mike@undisclosed.example.com) https://genetics.academic.csusb.edu/chapter_17?rev=1747457558&do=diff Chapter 16Table of ContentsChapter 18 Chapter 17. Newer tools for reverse genetics It is useful to briefly describe some newer technologies for reverse genetics that give researchers a bigger and more advanced toolbox for genetic analysis. Most of these tools are developed for use in mice, but generally they can be adapted to different model genetic organisms with some technical tweaks. $neo^R$$lox$$loxP$$loxP$$loxP$$loxP$$lox$$loxP$$loxP$$loxP$$loxP$$loxP$$neo^R$$loxP$$tk^{HSV}$$neo^R$$neo^R$$… text/html 2025-05-05T02:53:42+00:00 mike (mike@undisclosed.example.com) https://genetics.academic.csusb.edu/chapter_15?rev=1746413622&do=diff Chapter 14Table of ContentsChapter 16 Chapter 15. Transgenes in multicellular model organisms In the next two chapters we will examine some of the ways in which we can study gene function in higher eukaryotes, using the laboratory mouse Mus musculus as an example. A remarkable number of manipulations have been made to the mouse text/html 2025-04-29T18:56:36+00:00 mike (mike@undisclosed.example.com) https://genetics.academic.csusb.edu/chapter_14?rev=1745952996&do=diff Chapter 13Table of ContentsChapter 15 Chapter 14. Using reverse genetics to study molecules Introduction In this chapter we will see how genetics can be used to dissect molecular structure and function. We have seen one example of how genetics can achieve this in Chapter 13 with the $gal4^{81}$mutant - this mutant taught us something about how Gal80p functions in relation to Gal4p. In this chapter we will look at two further examples of how genetics can teach us about molecular function. In ou… text/html 2025-04-29T18:54:32+00:00 mike (mike@undisclosed.example.com) https://genetics.academic.csusb.edu/chapter_13?rev=1745952872&do=diff Chapter 12Table of ContentsChapter 14 Chapter 13. Gene regulation in eukaryotes Introduction In Chapter 12 we considered the structure of genes in eukaryotic organisms and discussed a general strategy for identifying and cloning S. cerevisiae genes that are transcriptionally regulated in response to a change in environment. The ability to regulate $GAL$$GAL4$$gal4$$gal4\text{-}1$$gal4$$gal1$$GAL4$$GAL4^+$$gal4^-$$gal1$$gal7$$gal10$$gal1$$gal1$$gal1$$GAL$$GAL$$lacZ$$GAL1$$GAL1$$gal1$$GAL1$$gal4… text/html 2025-04-15T13:57:30+00:00 mike (mike@undisclosed.example.com) https://genetics.academic.csusb.edu/chapter_12?rev=1744725450&do=diff Chapter 11Table of ContentsChapter 13 Chapter 12. Cloning regulated genes in eukaryotes For the last several chapters we have been looking at how one can study and manipulate prokaryotic genomes and how prokaryotic genes are regulated. In the next several chapters we will be considering eukaryotic genes and genomes and considering how model <a id="genome_compare"></a>RNA $lacZ$$lacZ$$lacZ$$lacZ$$lacZ$$lacO$$lacP$$URA3$$URA3$$tet^R$$URA3$$tet^R$$ura3$$URA3$$lacZ$$lacO$$lacP$$lacO$$lacP$$lacZ$$la… text/html 2024-11-26T02:22:26+00:00 mike (mike@undisclosed.example.com) https://genetics.academic.csusb.edu/?image=phase_known_graph.png&ns=&rev=1732587746&tab_details=history&media_do=diff&do=media <img src="https://genetics.academic.csusb.edu/_media/phase_known_graph.png?w=500&amp;h=500&amp;tok=692794" alt="phase_known_graph.png" loading="lazy" width="500" height="500" /> text/html 2024-11-26T01:20:31+00:00 mike (mike@undisclosed.example.com) https://genetics.academic.csusb.edu/?image=pedigree_ex2.jpg&ns=&rev=1732584031&tab_details=history&media_do=diff&do=media <img src="https://genetics.academic.csusb.edu/_media/pedigree_ex2.jpg?w=500&amp;h=500&amp;tok=552026" alt="pedigree_ex2.jpg" loading="lazy" width="500" height="500" 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