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glossary:primer [2024/08/31 16:26] – mike		glossary:primer [2024/08/31 16:27] (current) – mike
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-	Primer: (1) A short (usually 15-30 nt long, depending on application) that is synthesized in vitro and used in applications such as DNA sequencing and PCR. In this context, primers can also be called %%oligonucleotides%%. (2) a free 3'-OH end on a nucleic acid that can be used for initiating DNA synthesis.	+	Primer: (1) A short (usually 15-30 nt long, depending on application) piece of ssDNA that is synthesized in vitro and used in applications such as DNA sequencing and PCR. In this context, primers can also be called %%oligonucleotides%%. (2) a free 3'-OH end on a nucleic acid that can be used for initiating DNA synthesis.