chapter_02
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| chapter_02 [2026/10/25 00:52] – [The yeast lifecycle] mike | chapter_02 [2026/10/25 00:54] (current) – [Growing yeast in the lab] mike | ||
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| In yeast, haploids and diploids are isomorphic – meaning that if there is a change in a gene (i.e., a mutation ) that affects some function of the yeast cells, then essentially the same change will be observed in both haploid and diploid cells. This allows us to look at the effect of having two different alleles (i.e., versions) of the same gene in the same diploid cell. For instance, a haploid yeast cell might carry a mutant allele of the $his3$ gene, whereas a diploid might carry two identical $his3$/ | In yeast, haploids and diploids are isomorphic – meaning that if there is a change in a gene (i.e., a mutation ) that affects some function of the yeast cells, then essentially the same change will be observed in both haploid and diploid cells. This allows us to look at the effect of having two different alleles (i.e., versions) of the same gene in the same diploid cell. For instance, a haploid yeast cell might carry a mutant allele of the $his3$ gene, whereas a diploid might carry two identical $his3$/ | ||
| - | All yeast needs to grow in the lab (other than water and warmth) are salts, minerals, and glucose; growth media that contains only these things is called minimal media. Using the compounds contained in minimal media, normal yeast cells can synthesize all the molecules that are needed to construct a cell, such as amino acids (including histidine) and nucleotides. Organisms that are able to do this are called prototrophs. In a laboratory context, we define normal yeast cells that can grow on minimal media as wildtype. Note that " | + | All that yeast needs to grow in the lab (other than water and warmth) are salts, minerals, and an organic carbon source (usually a sugar, but even glycerol can work!); growth media that contains only these things is called minimal media. Using the compounds contained in minimal media, normal yeast cells can synthesize all the molecules that are needed to construct a cell, such as amino acids (including histidine) and nucleotides. Organisms that are able to do this are called prototrophs. In a laboratory context, we define normal yeast cells that can grow on minimal media as wildtype. Note that " |
| ===== Genes are functionally defined by mutations that alter their function ===== | ===== Genes are functionally defined by mutations that alter their function ===== | ||
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