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--- glossary:crispr [2024/09/01 21:05] – created mike
+++ glossary:crispr [2024/09/01 21:26] (current) – mike
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-CRISPR: short for clustered regularly interspaced short palindromic repeats. In bacteria, CRISPR is a form of innate immunity that bacterial cells use as a defense against bacteriophage. The CRISPR system consists of a protein typically called Cas9 and two non-coding RNAs called gRNA and tracrRNA. Scientists have co-opted it as a research tool that can be used to cleave dsDNA at nearly any desired location with a high degree of specificity.
+%%CRISPR%%: short for clustered regularly interspaced short palindromic repeats. In bacteria, %%CRISPR%% is a form of innate immunity that bacterial cells use as a defense against bacteriophage. The %%CRISPR%% system consists of a protein typically called %%Cas9%% and two non-coding RNAs called %%gRNA%% and tracrRNA. The %%gRNA%% directs the %%CRISPR/Cas9%% enzyme to a DNA target based on base pairing between the %%gRNA%% sequence and the target DNA.
+Scientists have co-opted the %%CRISPR/Cas9%% system as a research tool that can be used to cleave dsDNA at nearly any desired location with a high degree of specificity. They do this by modifying the %%gRNA%% sequence to match a desired target DNA.