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CRISPRplugin-autotooltip__default plugin-autotooltip_bigCRISPR: short for clustered regularly interspaced short palindromic repeats. In bacteria, CRISPR is a form of innate immunity that bacterial cells use as a defense against bacteriophage. The CRISPR system consists of a protein typically called Cas9 and two non-coding RNAs called gRNA and tracrRNA. The: short for clustered regularly interspaced short palindromic repeats. In bacteriaplugin-autotooltip__default plugin-autotooltip_bigBacteria: Single-celled organisms that also utilize DNA and the standard genetic code as all organisms on earth, but unlike eukaryotes do not have intracellular membranes and membrane-bound organelles. In this book we use bacteria and prokaryote interchangeably., CRISPRplugin-autotooltip__default plugin-autotooltip_bigCRISPR: short for clustered regularly interspaced short palindromic repeats. In bacteria, CRISPR is a form of innate immunity that bacterial cells use as a defense against bacteriophage. The CRISPR system consists of a protein typically called Cas9 and two non-coding RNAs called gRNA and tracrRNA. The is a form of innate immunity that bacterialplugin-autotooltip__default plugin-autotooltip_bigBacteria: Single-celled organisms that also utilize DNA and the standard genetic code as all organisms on earth, but unlike eukaryotes do not have intracellular membranes and membrane-bound organelles. In this book we use bacteria and prokaryote interchangeably. cells use as a defense against bacteriophageplugin-autotooltip__default plugin-autotooltip_bigBacteriophage: viruses that infect bacteria.. The CRISPRplugin-autotooltip__default plugin-autotooltip_bigCRISPR: short for clustered regularly interspaced short palindromic repeats. In bacteria, CRISPR is a form of innate immunity that bacterial cells use as a defense against bacteriophage. The CRISPR system consists of a protein typically called Cas9 and two non-coding RNAs called gRNA and tracrRNA. The system consists of a proteinplugin-autotooltip__default plugin-autotooltip_bigProtein: a molecule that is formed by the translation of messenger RNAs (mRNAs). Functions that proteins provide are what usually give organisms their phenotypes. typically called Cas9plugin-autotooltip__default plugin-autotooltip_bigCRISPR: short for clustered regularly interspaced short palindromic repeats. In bacteria, CRISPR is a form of innate immunity that bacterial cells use as a defense against bacteriophage. The CRISPR system consists of a protein typically called Cas9 and two non-coding RNAs called gRNA and tracrRNA. The and two non-coding RNAsplugin-autotooltip__default plugin-autotooltip_bigRNA sequencing (RNAseq): an experimental technique that sequences all the RNAs in a sample. It is based off of converting RNAs into cDNAs with reverse transcriptase, followed by Illumina sequencing. called gRNAplugin-autotooltip__default plugin-autotooltip_bigCRISPR: short for clustered regularly interspaced short palindromic repeats. In bacteria, CRISPR is a form of innate immunity that bacterial cells use as a defense against bacteriophage. The CRISPR system consists of a protein typically called Cas9 and two non-coding RNAs called gRNA and tracrRNA. The and tracrRNA. The gRNAplugin-autotooltip__default plugin-autotooltip_bigCRISPR: short for clustered regularly interspaced short palindromic repeats. In bacteria, CRISPR is a form of innate immunity that bacterial cells use as a defense against bacteriophage. The CRISPR system consists of a protein typically called Cas9 and two non-coding RNAs called gRNA and tracrRNA. The directs the CRISPRplugin-autotooltip__default plugin-autotooltip_bigCRISPR: short for clustered regularly interspaced short palindromic repeats. In bacteria, CRISPR is a form of innate immunity that bacterial cells use as a defense against bacteriophage. The CRISPR system consists of a protein typically called Cas9 and two non-coding RNAs called gRNA and tracrRNA. The/Cas9plugin-autotooltip__default plugin-autotooltip_bigCRISPR: short for clustered regularly interspaced short palindromic repeats. In bacteria, CRISPR is a form of innate immunity that bacterial cells use as a defense against bacteriophage. The CRISPR system consists of a protein typically called Cas9 and two non-coding RNAs called gRNA and tracrRNA. The enzymeplugin-autotooltip__default plugin-autotooltip_bigEnzyme: a macromolecule, usually a protein (but sometimes an RNA), that functions as a catalyst of some kind of biochemical reaction. to a DNAplugin-autotooltip__default plugin-autotooltip_bigDNA: deoxyribonucleic acid. The genetic material for nearly all life on Earth. target based on base pairingplugin-autotooltip__default plugin-autotooltip_bigBase pair: a term used to describe how nitrogenous bases (G, A, T/U, and C) in nucleic acids interact with each other via hydrogen bonds to form double-stranded molecules (including dsDNA, dsRNA, and DNA/RNA hybrids). G always pairs with C, and T/U always pairs with A. between the gRNAplugin-autotooltip__default plugin-autotooltip_bigCRISPR: short for clustered regularly interspaced short palindromic repeats. In bacteria, CRISPR is a form of innate immunity that bacterial cells use as a defense against bacteriophage. The CRISPR system consists of a protein typically called Cas9 and two non-coding RNAs called gRNA and tracrRNA. The sequenceplugin-autotooltip__default plugin-autotooltip_bigSequence: the precise order of monomers in a polymer. In DNA, it refers to the order of G, A, T, and C nucleotides. In RNA, it refers to the order of G, A, U, and C nucleotides. In proteins, it refers to the order of amino acids. and the target DNAplugin-autotooltip__default plugin-autotooltip_bigDNA: deoxyribonucleic acid. The genetic material for nearly all life on Earth..
Scientists have co-opted the CRISPRplugin-autotooltip__default plugin-autotooltip_bigCRISPR: short for clustered regularly interspaced short palindromic repeats. In bacteria, CRISPR is a form of innate immunity that bacterial cells use as a defense against bacteriophage. The CRISPR system consists of a protein typically called Cas9 and two non-coding RNAs called gRNA and tracrRNA. The/Cas9plugin-autotooltip__default plugin-autotooltip_bigCRISPR: short for clustered regularly interspaced short palindromic repeats. In bacteria, CRISPR is a form of innate immunity that bacterial cells use as a defense against bacteriophage. The CRISPR system consists of a protein typically called Cas9 and two non-coding RNAs called gRNA and tracrRNA. The system as a research tool that can be used to cleave dsDNAplugin-autotooltip__default plugin-autotooltip_bigDouble-stranded DNA (dsDNA): DNA that consists of two complementary strands of ssDNA paired together via hydrogen bonds between the nitrogenous bases G, A, T, and C. at nearly any desired location with a high degree of specificity. They do this by modifying the gRNAplugin-autotooltip__default plugin-autotooltip_bigCRISPR: short for clustered regularly interspaced short palindromic repeats. In bacteria, CRISPR is a form of innate immunity that bacterial cells use as a defense against bacteriophage. The CRISPR system consists of a protein typically called Cas9 and two non-coding RNAs called gRNA and tracrRNA. The sequenceplugin-autotooltip__default plugin-autotooltip_bigSequence: the precise order of monomers in a polymer. In DNA, it refers to the order of G, A, T, and C nucleotides. In RNA, it refers to the order of G, A, U, and C nucleotides. In proteins, it refers to the order of amino acids. to match a desired target DNAplugin-autotooltip__default plugin-autotooltip_bigDNA: deoxyribonucleic acid. The genetic material for nearly all life on Earth..