Plasmid: a circular episomeplugin-autotooltip__default plugin-autotooltip_bigEpisome: a piece of DNA that exists separately from chromosomes and that can replicate and segregate during cell division. While all episomes are natural in origin, they are commonly exploited by researchers for genetics research. found in bacteriaplugin-autotooltip__default plugin-autotooltip_bigBacteria: Single-celled organisms that also utilize DNA and the standard genetic code as all organisms on earth, but unlike eukaryotes do not have intracellular membranes and membrane-bound organelles. In this book we use bacteria and prokaryote interchangeably. and yeastplugin-autotooltip__default plugin-autotooltip_bigYeast: in this book, refers to Saccharomyces cerevisiae, a single-celled eukaryotic microbe used as a model genetic organism. See Chapter 02. Plasmids are commonly used as cloningplugin-autotooltip__default plugin-autotooltip_bigClone: Depending on the context, this word can have a few different meanings:
* In the context of genes, cloning means that the physical identity of a gene has been found, and the gene has been sequenced. * In the context of DNA, a cloned DNA fragment is one that has been inserted into some kind of vectorsplugin-autotooltip__default plugin-autotooltip_bigVector: in molecular genetics, a vector is a genetic element (usually an episome of sort such as a plasmid but can be something like a virus) that is used to clone and/or transfer genes into and out of cells..
