Two hybrid system: an experimental procedure, usually performed in yeastplugin-autotooltip__default plugin-autotooltip_bigYeast: in this book, refers to Saccharomyces cerevisiae, a single-celled eukaryotic microbe used as a model genetic organism. See Chapter 02, that leverages our knowledge of how transcriptionplugin-autotooltip__default plugin-autotooltip_bigRNA transcription: the process of RNA polymerase using the DNA sequence of a gene as a template to form an mRNA (in prokaryotes) or pre-mRNA (in eukaryotes). In most cases, “transcription” implies RNA transcription. works in the $GAL4$-UASplugin-autotooltip__default plugin-autotooltip_bigUpstream activator sequence (UAS): In a general sense, a UAS is a cis-acting regulatory element, usually located significantly upstream of the promoter and TATA box, to which transactivators bind to activate transcription of nearby genes.
Although the term “UAS” can in principle be used to describe any cis-acting upstream sequence that activates transcription, in practice the term enhancer is used more often to describe these $GAL1$ system. It is a general test that can be used to determine if two proteinsplugin-autotooltip__default plugin-autotooltip_bigProtein: a molecule that is formed by the translation of messenger RNAs (mRNAs). Functions that proteins provide are what usually give organisms their phenotypes. physically interact with each other.
glossary:two_hybrid_system
glossary/two_hybrid_system.txt · Last modified: 2024/09/01 18:41 by mike