DNA ligaseplugin-autotooltip__default plugin-autotooltip_bigDNA ligase: an commercially available enzyme used to form phosphodiester bonds between the 3' end of one strand of dsDNA and the 5'end of another. Used for molecular cloning experiments. Its natural function in cells relates to lagging strand DNA synthesis and various DNA repair mechanisms.: an commercially available enzymeplugin-autotooltip__default plugin-autotooltip_bigEnzyme: a macromolecule, usually a protein (but sometimes an RNA), that functions as a catalyst of some kind of biochemical reaction. used to form phosphodiester bonds between the 3' end of one strand of dsDNAplugin-autotooltip__default plugin-autotooltip_bigDouble-stranded DNA (dsDNA): DNA that consists of two complementary strands of ssDNA paired together via hydrogen bonds between the nitrogenous bases G, A, T, and C. and the 5'end of another. Used for molecular cloningplugin-autotooltip__default plugin-autotooltip_bigClone: Depending on the context, this word can have a few different meanings:
* In the context of genes, cloning means that the physical identity of a gene has been found, and the gene has been sequenced. * In the context of DNA, a cloned DNA fragment is one that has been inserted into some kind of experiments. Its natural function in cells relates to lagging strand DNAplugin-autotooltip__default plugin-autotooltip_bigDNA: deoxyribonucleic acid. The genetic material for nearly all life on Earth. synthesis and various DNAplugin-autotooltip__default plugin-autotooltip_bigDNA: deoxyribonucleic acid. The genetic material for nearly all life on Earth. repair mechanisms.